Evaluation of The Specificity of Diagnostic Hyperimmune Serum Produced Against Brucellosis in Heterologous Microorganisms

Akmal Pulatovich Yusupov; Otabek Kuziev Jurakulovich

doi:10.37547/ijmsphr/Volume07Issue02-16

Authors

Akmal Pulatovich Yusupov

Alfraganus University, Department of Medicine, PhD (Doctor of Philosophy), Associate Professor, Uzbekistan

https://orcid.org/0009-0007-4167-5433
Otabek Kuziev Jurakulovich

Alfraganus University, Head of the Department of Medicine, DSc, Professor, Uzbekistan

https://orcid.org/0000-0002-7277-2224

Keywords

Brucellosis Brucella abortus 19 hyperimmune serum

Abstract

The production of high-quality diagnostic reagents is essential for accurate serological diagnosis of brucellosis. This study aimed to evaluate the specificity of hyperimmune sera obtained by hyperimmunization of rabbits with the live Brucella abortus 19 (BA19) vaccine strain. The specificity was tested against a wide panel of clinical heterologous microorganisms from the Enterobacteriaceae family commonly associated with intestinal infections. Although weak positive reactions were observed in the slide agglutination test with a limited number of strains (Salmonella spp., Escherichia coli, and Citrobacter spp.), all reactions were negative in the confirmatory tube agglutination test. The results confirm the high specificity of the obtained diagnostic sera and support their reliable application in laboratory practice for the serological identification of Brucella pathogens.

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